07 Nov Environmental DNA – Plasmid DNA as optimal positive controls
November 12th from 12-1pm ET for the latest installment of the NOAA Innovators Series with Dr. John Wood of Pisces Molecular LLC!
Register using this link: https://attendee.gotowebinar.com/register/5863990582442113283
Speaker: Dr. John Wood, Pisces Molecular LLC (presenting remotely)
Abstract: We have developed plasmid DNA based qPCR positive standards for a variety of molecular environmental assays. Plasmid standards provide significant benefits over more typically used genomic DNA standards: (I ) Target sequence copy numbers can be more accurately quantified. (2) Multiple targets can be incorporated in one plasmid. (3) They are distinguishable from the native sequence, eliminating the danger of false positives in test samples due to contamination from the standards. (4) They can be made in nearly unlimited quantity.
About the Speaker: Dr. Wood has carried out molecular genetics research in a variety of areas including yeast cell division control, human disease gene mapping, recombinant hemoglobin as a blood substitute, and after founding Pisces Molecular some years ago, problems in wildlife, conservation and the environment.
Accessibility: *This presentation will be recorded and available on our YouTube Channel. If you would like to request an ASL interpreter in person or via webcam for an upcoming webinar, please apply through the NOAA Office of Human Capital Services’ Sign Language Interpreting Services Program.